EXPERTISE

Physico-chemical characterization of (bio)molecular complexes and (bio)molecular interactions, using UV-Vis spectroscopy, microcalorimetry and NMR spectroscopy. 
This expertise is extremely valuable for the pharmaceutical industry, for the development of smart materials and molecular sensors.
Use of micelles and liposomes for the solubilisation of organic receptors and characterization of these supramolecular assemblies (DSC, NMR: 1D-NOE, PRE, …).
This expertise is extremely valuable for the pharmaceutical industry and for the development of molecular sensors that work in an aqueous environment.
Synthesis of nanoparticles of different nature (gold, silver, silica, iron oxide), characterization and functionalization using organic ligands and biomolecules.
This expertise is extremely valuable in the context of the development of sensors, separation techniques, smart materials and drug delivery systems.
Preparation of liposomes and their application in the study of transmembrane transport of ions using fluorescence and NMR spectroscopy.
This expertise is extremely valuable in the development of transmembrane transporters for biomedical applications.

EQUIPMENT

Isothermal Titration Calorimetry


Two nano-ITC systems from TA instruments, one ITC 4200 with 1.3 ml removable cells and a Nano-ITC 2G with 1 ml non-removable cells.  These systems are ideal for the characterization of intermolecular interactions, from small organic molecules to biological macromolecules, and can be used both with aqueous or organic solutions.

Differential Scanning Calorimetry
A nano-DSC III from TA instruments with a 300 μl capillary cell specifically designed to determine the thermal stability and heat capacity of proteins and other macromolecules or macromolecular assemblies (liposomes) in dilute solution.
UV-Vis-NIR Absorption Spectrophotometer


Equipped with a Pelletier Temperature Controller and an Integration Sphere allowing the characterization of low volume liquid samples (150 μl) and solid samples from 200 to 3500 nm.

Emmission Spectrophotometer


Fluoromax-4 equipped with an injection port, stirrer, and temperature control.

Dynamic Light Scattering


Malvern Zetasizer Ultra for size (3 angles) and zetapotential measurements.

High Resolution NMR Spectrometers

The EMNS laboratory has access, via the CIREM, to three High Resolution NMR spectrometers (600, 400 and 300 MHz) equipped with different probes for solution studies.

RMN

Updated on August 5, 2021