Published on October 13, 2023
– Updated on October 13, 2023
by Matúš Chvojka, Anurag Singh, Alessio Cataldo, Aaron Torres-Huerta, Marcin Konopka, Vladimír Šindelář, Hennie Valkenier First published: 7 August 2023
The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles
Graphical Abstract
The lucigenin assay is a tool to study the transmembrane transport of chloride and other anions by synthetic anion transporters using fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay, including detailed procedures, advice on troubleshooting, and variations to study different anion transport processes.